Journal: Frontiers in Bioengineering and Biotechnology

Article Title: pH-responsive theranostic nanoplatform of ferrite and ceria co-engineered nanoparticles for anti-inflammatory

doi: 10.3389/fbioe.2022.983677

Figure Lengend Snippet: Morphological and compositional characterizations of Fe/Ce-MSN NPs: (A–C) TEM, SEM images and XRD patterns of Fe/Ce-MSN NPs. (D–G) Elemental mapping of Fe/Ce-MSN: Fe, Ce, Si, and O. h) XPS spectra of Fe 2p. (I,J) XPS spectra of Ce 3d before and after introduce Fe ions. (K) The content ratio of Ce 3+ and Ce 4+ from the XPS spectra in (I,J) . (L) Typical particle size distribution diagrams of Fe/Ce-MSN and Fe/Ce-MSN-PEG NPs. (M) the physical stability of Fe/Ce-MSN-PEG NPs at 37°C in serum (5%, pH 7.4). The hydrodynamic size of Fe/Ce-MSN-PEG NPs was determined over 1 week.

Article Snippet: The morphology of Fe/Ce-MSNs was observed with transmission electron microscopy (TEM) (TECNAI-10 microscope, Philips, Netherlands) and Scanning electron microscopy (SEM) (FIB-SEM microscope, Crossbeam 340, Zeiss).

Techniques: Introduce

Journal: Journal of Advanced Research

Article Title: Antibody-antibiotic conjugate targeted therapy for orthopedic implant-associated intracellular S. aureus infections

doi: 10.1016/j.jare.2023.12.001

Figure Lengend Snippet: Intracellular S. aureus infection resists antibiotic treatment. A) Image of bacterial plate coating of cell suspension and cytoplasm; B) Confocal microscopy showed the growth of bacteria in RAW264.7 (red) infected by S. aureus (green) at different time points in the presence of antibiotics (scale bar = 10 µm). C) Analysis of intracellular fluorescence intensity of S. aureus at different time points Analysis of intracellular fluorescence intensity of S. aureus at different time points (ns, no statistical difference; **, p < 0.01). D) Mice knee tissue bacterial load, 7 days after infection with equal amounts of intracellular bacteria and planktonic bacteria (C.F.U., colony-forming units, n = 5 mice per group, ***, p < 0.001). (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)

Article Snippet: Scanning electron microscopy (SEM, Zeiss FIB-SEM, Crossbeam 340, Germany) was used to visualize biofilm formation on implant surface.

Techniques: Infection, Suspension, Confocal Microscopy, Bacteria, Fluorescence

Journal: Journal of Advanced Research

Article Title: Antibody-antibiotic conjugate targeted therapy for orthopedic implant-associated intracellular S. aureus infections

doi: 10.1016/j.jare.2023.12.001

Figure Lengend Snippet: The efficacy of AAC in the treatment of S. aureus intracellular infection assessed by histology and scanning electron microscopy (SEM). A) Hematoxylin and eosin staining (H&E) of joint tissues and B) Gram stain. C) Representative SEM images show biofilm formation on the internal fixation surface in each group (Yellow triangular arrows marked S. aureus , Scale bar, 1 mm, n = 5 per group). (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)

Article Snippet: Scanning electron microscopy (SEM, Zeiss FIB-SEM, Crossbeam 340, Germany) was used to visualize biofilm formation on implant surface.

Techniques: Infection, Electron Microscopy, Staining